Anticorps Polyclonal de lapin anti-RBBP6

• Phare
• Validé par KD/KO

RBBP6 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11882-1-PBS

Synonymes

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

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Galerie de données de validation

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  WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  WB analysis using 11882-1-AP (same clone as 11882-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11882-1-AP (RBBP6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11882-1-AP (same clone as 11882-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11882-1-AP (RBBP6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11882-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11882-1-PBS cible RBBP6 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBBP6 Protéine recombinante Ag2484
• Nom complet: retinoblastoma binding protein 6
• Masse moléculaire calculée: 1792 aa, 200 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC029352
• Symbole du gène: RBBP6
• Identification du gène (NCBI): 5930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

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