Anticorps Recombinant de lapin anti-RBBP6

RBBP6 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250142C1

N° de cat : 85884-5-RR

Synonymes

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

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Galerie de données de validation

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  WB analysis using 85884-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85884-5-RR (RBBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 85884-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RBBP6 antibody (85884-5-RR, Clone: 250142C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85884-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85884-5-RR against Human RBBP6 were performed. The affinity constant is 0.384 nM.

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Applications testées

• Résultats positifs en WB: cellules EC109, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85884-5-RR cible RBBP6 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBBP6 Protéine recombinante Ag2484
• Nom complet: retinoblastoma binding protein 6
• Masse moléculaire calculée: 1792 aa, 200 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC029352
• Symbole du gène: RBBP6
• Identification du gène (NCBI): 5930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

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