Anticorps Recombinant de lapin anti-RbAp46

RbAp46 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241189D6

N° de cat : 84330-1-RR

Synonymes

RBBP7, 241189D6, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7

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Galerie de données de validation

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  WB analysis using 84330-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84330-1-RR (RBBP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84330-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84330-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 84330-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 84330-1-RR

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84330-1-RR (RBBP7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 84330-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBBP7 antibody (84330-1-RR, Clone: 241189D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 84330-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RBBP7 Recombinant antibody (84330-1-RR, Clone:241189D6) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y
• Résultats positifs en IHC: human rectal cancer tissue, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84330-1-RR cible RbAp46 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: retinoblastoma binding protein 7
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_002893
• Symbole du gène: RBBP7
• Identification du gène (NCBI): 5931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

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