Anticorps Polyclonal de lapin anti-RBBP9

• Phare
• Validé par KD/KO

RBBP9 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12230-2-AP

Synonymes

B5T overexpressed gene protein, BOG, Protein BOG, Putative hydrolase RBBP9, RBBP 10, RBBP 9, RBBP10, RBBP9

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Galerie de données de validation

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  WB analysis of mouse testis using 12230-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12230-2-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12230-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12230-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12230-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12230-2-AP (RBBP9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 12230-2-AP

  IP Result of anti-RBBP9 (IP:12230-2-AP, 3ug; Detection:12230-2-AP 1:300) with mouse lung tissue lysate 5160ug.

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  IHC staining of human pancreas cancer using 12230-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12230-2-AP (RBBP9 antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of BxPC-3 using 12230-2-AP

  1X10^6 BxPC-3 cells were stained with 0.2ug RBBP9 antibody (12230-2-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules A375, tissu pulmonaire de souris, tissu pulmonaire humain, tissu splénique humain
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12230-2-AP cible RBBP9 dans les applications de WB, IP, IHC, IF, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBBP9 Protéine recombinante Ag2868
• Nom complet: retinoblastoma binding protein 9
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC015938
• Symbole du gène: RBBP9
• Identification du gène (NCBI): 10741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.

Publications

Species	Application	Title
human	WB,IHC,IF	Proc Natl Acad Sci U S A; RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia.; Authors - Shields David J DJ
human,mouse	WB	Antioxid Redox Signal; Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins.; Authors - Maya A Olshina
human	WB	Exp Hematol; Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance.; Authors - Michael D O'Connor ; KD Validated
human	WB	Dis Markers; Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis.; Authors - Nachmany Henny H

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