Anticorps Monoclonal anti-RBBP9

• Phare
• Validé par KD/KO

RBBP9 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

3D5E11

N° de cat : 66015-2-Ig

Synonymes

BOG, RBBP9

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Galerie de données de validation

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  WB analysis using 66015-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66015-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66015-2-Ig

  WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.

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  WB analysis using 66015-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66015-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66015-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66015-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 66015-2-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 66015-2-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed A549 cells using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66015-2-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral de porc, cellules A375, cellules A549, cellules BxPC-3, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66015-2-Ig cible RBBP9 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RBBP9 Protéine recombinante Ag17864
• Nom complet: retinoblastoma binding protein 9
• Masse moléculaire calculée: 186 aa, 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC015938
• Symbole du gène: RBBP9
• Identification du gène (NCBI): 10741
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)