Anticorps Recombinant de lapin anti-RBM20

RBM20 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240345B7

N° de cat : 83363-1-PBS

Synonymes

240345B7, RNA binding motif protein 20, RNA binding protein

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Galerie de données de validation

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  WB analysis using 83363-1-RR (same clone as 83363-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83363-1-RR (RBM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00379-4

  Sandwich ELISA standard curve of MP00379-4, Human RBM20 Recombinant Matched Antibody Pair - PBS only. 83363-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34322. 83363-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00379-4

  The mean RBM20 concentration was determined to be 1.8 ng/mL in A549 cell extract based on a 2.0 mg/mL extract load.

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  FC experiment of K-562 using 83363-1-RR (same clone as 83363-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.2 ug RBM20 Recombinant antibody (83363-1-RR, Clone:240345B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83363-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83363-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83363-1-RR against Human RBM20 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83363-1-PBS cible RBM20 dans les applications de WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBM20 Protéine recombinante Ag34322
• Nom complet: RNA binding motif protein 20
• Masse moléculaire calculée: 134 kDa
• Poids moléculaire observé: 190 kDa
• Numéro d’acquisition GenBank: NM_001134363
• Symbole du gène: RBM20
• Identification du gène (NCBI): 282996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBM20 (RNA binding motif protein 20) is a heart- and skeletal muscle- nriched splicing factor that controls tissue-specific isoform expression of many other genes besides TTN, including sarcomeric genes such myomesin 1, but also Ca2+- and ion-handling genes such as CAMK2D (calcium/calmodulin-dependent kinase II-delta) and RYR2 (ryanodine receptor 2) (PMID: 29650543).

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