Anticorps Polyclonal de lapin anti-RBM22

RBM22 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22103-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Raji using 22103-1-AP (same clone as 22103-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

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  WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 22103-1-AP (same clone as 22103-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

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Informations sur le produit

22103-1-PBS cible RBM22 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM22 Protéine recombinante Ag17315
• Nom complet: RNA binding motif protein 22
• Masse moléculaire calculée: 420 aa, 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC003402
• Symbole du gène: RBM22
• Identification du gène (NCBI): 55696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

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