Anticorps Recombinant de lapin anti-RBM25

RBM25 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242526B7

N° de cat : 84968-4-PBS

Synonymes

242526B7, Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25

---

Galerie de données de validation

• 

  WB analysis using 84968-4-RR (same clone as 84968-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84968-4-RR (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84968-4-RR (same clone as 84968-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84968-4-RR (RBM25 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84968-4-RR (same clone as 84968-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (84968-4-RR, Clone: 242526B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84968-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84968-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84968-4-RR against Human RBM25 were performed. The affinity constant is 2.38 nM.

---

Informations sur le produit

84968-4-PBS cible RBM25 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBM25 Protéine recombinante Ag18244
• Nom complet: RNA binding motif protein 25
• Masse moléculaire calculée: 843 aa, 100 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC113389
• Symbole du gène: RBM25
• Identification du gène (NCBI): 58517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

{{ptg:RelatedPrimaryAntibodies}}