Anticorps Monoclonal anti-RBM4

• Phare
• Validé par KD/KO

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H3F12

N° de cat : 60292-1-PBS

Synonymes

3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4

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Galerie de données de validation

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  WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

  WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60292-1-Ig (same clone as 60292-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60292-1-PBS cible RBM4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RBM4 Protéine recombinante Ag2190
• Nom complet: RNA binding motif protein 4
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC021120
• Symbole du gène: RBM4
• Identification du gène (NCBI): 5936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

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