Anticorps Recombinant de lapin anti-RBM47

RBM47 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250436H8

N° de cat : 86009-1-PBS

Synonymes

NET18, RNA binding motif protein 47, RNA binding protein 47, RNA-binding motif protein 47, RNA-binding protein 47

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Galerie de données de validation

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  WB analysis using 86009-1-RR (same clone as 86009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86009-1-RR (RBM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86009-1-RR (same clone as 86009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 86009-1-RR (same clone as 86009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 86009-1-RR (RBM47 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 86009-1-RR (same clone as 86009-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM47 antibody (86009-1-RR, Clone: 250436H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86009-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86009-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86009-1-RR against Human RBM47 were performed. The affinity constant is 1.02 nM.

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Informations sur le produit

86009-1-PBS cible RBM47 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBM47 Protéine recombinante Ag20984
• Nom complet: RNA binding motif protein 47
• Masse moléculaire calculée: 593 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC126261
• Symbole du gène: RBM47
• Identification du gène (NCBI): 54502
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBM47, also known as RNA-binding motif protein 47 or RNA-binding protein 47, is a vertebrate-conserved RBP containing three RNA-recognition motifs (RRMs) that perform multifaceted roles in RNA editing, early embryonic development, and cancer.Recent studies suggest that RBM47 functions as a tumor suppressor. It has been shown to suppress the progression of breast and colon cancers. RBM47 represents a molecular switch of cell fate decisions that functions as a regulator of the p53/p21-signaling axis. (PMID: 34499322, PMID: 31511650)

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