Anticorps Polyclonal de lapin anti-RBM6

RBM6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14360-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14360-1-AP (same clone as 14360-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse spleen using 14360-1-AP (same clone as 14360-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14360-1-AP (RBM6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

  IP result of anti-RBM6 (IP:14360-1-AP, 4ug; Detection:14360-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 14360-1-AP (same clone as 14360-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14360-1-AP (RBM6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14360-1-AP (same clone as 14360-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14360-1-AP (RBM6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14360-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14360-1-PBS cible RBM6 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM6 Protéine recombinante Ag5743
• Nom complet: RNA binding motif protein 6
• Masse moléculaire calculée: 129 kDa
• Poids moléculaire observé: 129 kDa
• Numéro d’acquisition GenBank: BC046643
• Symbole du gène: RBM6
• Identification du gène (NCBI): 10180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBM6, also named as DEF3, is a 1123 amino acid protein, which may interact with FAM168B. RBM6 is expressed ubiquitously in adults tissues. RBM6 specifically binds poly(G) RNA homopolymers in vitro. RBM6 exists some isoforms with MV 129 kDa, 69 kDa, and 59 kDa. onversely, fetal thymus and adult kidney express very little RBM6. In the hemopoietic system of mice, the expression of RBM6 is downregulated upon differentiation of progenitor cells into granulocytes but persists during macrophage development.

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