Anticorps Polyclonal de lapin anti-RBM7

RBM7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 21896-1-AP

Synonymes

RNA-binding protein 7, RNA-binding motif protein 7, RNA binding protein 7, RNA binding motif protein 7



Applications testées

Résultats positifs en WBcellules HepG2, cellules HeLa
Résultats positifs en IHCtissu de cancer du foie humain, tissu de cancer du poumon humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

21896-1-AP cible RBM7 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène RBM7 Protéine recombinante Ag11106
Nom complet RNA binding motif protein 7
Masse moléculaire calculée 266 aa, 31 kDa
Poids moléculaire observé 30-35 kDa
Numéro d’acquisition GenBankBC034381
Symbole du gène RBM7
Identification du gène (NCBI) 10179
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM7 (RNA-binding motif protein 7) is an RNA-binding protein initially described to associate with splicing factors, presumably to target intronic RNA for decay (PMID: 35688134). RBM7 regulates the nuclear degradation of NEAT1 non-coding RNA, resulting in sustained apoptosis in the lung epithelium and fibrosis (PMID: 32708315).

Protocole

Product Specific Protocols
WB protocol for RBM7 antibody 21896-1-APDownload protocol
IHC protocol for RBM7 antibody 21896-1-APDownload protocol
IF protocol for RBM7 antibody 21896-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Temporal-iCLIP captures co-transcriptional RNA-protein interactions

Authors - Ross A Cordiner
humanWB

Mol Cell

ARS2 instructs early transcription termination-coupled RNA decay by recruiting ZC3H4 to nascent transcripts

Authors - Jérôme O Rouvière
humanWB

Nucleic Acids Res

The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs

Authors - Qianqian Yang
  • KD Validated
humanWB

Mol Cell

P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.

Authors - Andrii Bugai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Aleksandr (Verified Customer) (12-16-2024)

Specific antibody!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: RKO
RBM7 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:21896-1-AP)
FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody that detect Rbm7 in U2OS cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: U2OS
RBM7 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:21896-1-AP)
FH

Moritz (Verified Customer) (08-20-2024)

I used this antibody to detect RBM7 with Western blot in HEK293T WT cell lysate, which worked perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HEK293T WT
RBM7 Antibody Western Blot validation (1:3000 dilution) in HEK293T WT (Cat no:21896-1-AP)
FH

Roy (Verified Customer) (06-15-2024)

Works well to detect the NEXT complex subunit RBM7 from HeLa extracts (1/1000 Dilution, overnight Incubation at 4°C).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
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