Anticorps Recombinant de lapin anti-RBMS1

RBMS1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240677D6

N° de cat : 83623-5-RR

Synonymes

MSSP 2, MSSP 1, MSSP, HCC 4, C2orf12

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Galerie de données de validation

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  WB analysis using 83623-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83623-5-RR (RBMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 83623-5-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBMS1 Recombinant antibody (83623-5-RR, Clone:240677D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83623-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83623-5-RR against Human RBMS1 were performed. The affinity constant is 0.726 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83623-5-RR cible RBMS1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBMS1 Protéine recombinante Ag1493
• Nom complet: RNA binding motif, single stranded interacting protein 1
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC018951
• Symbole du gène: RBMS1
• Identification du gène (NCBI): 5937
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBMS1 (RNA binding motif single stranded interacting protein 1), also known as C2orf12. It is expected to be located in the nucleus, which is ubiquitinated in placenta and endometrium. Highest amounts are found in placenta, lung and heart. This gene encodes a member of a small family of proteins which bind single stranded DNA/RNA. These proteins are characterized by the presence of two sets of ribonucleoprotein consensus sequence (RNP-CS) that contain conserved motifs, RNP1 and RNP2, originally described in RNA binding proteins, and required for DNA binding. These proteins have been implicated in such diverse functions as DNA replication, gene transcription, cell cycle progression and apoptosis. The molecular weight of RBMS1 is 45 kDa.

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