Anticorps Polyclonal de lapin anti-RBP1

• Phare
• Validé par KD/KO

RBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 22683-1-AP

Synonymes

Cellular retinol-binding protein, Cellular retinol-binding protein I, CRABP I, CRBP I, CRBP1

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Galerie de données de validation

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  WB analysis using 22683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 22683-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22683-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22683-1-AP (RBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22683-1-AP (RBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 22683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 22683-1-AP (RBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 22683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RBP1 antibody (22683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, tissu cérébral humain, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du côlon humain, tissu hépatique humain, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

22683-1-AP cible RBP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBP1 Protéine recombinante Ag18572
• Nom complet: retinol binding protein 1, cellular
• Masse moléculaire calculée: 135 aa, 16 kDa
• Poids moléculaire observé: 14-21 kDa
• Numéro d’acquisition GenBank: BC121052
• Symbole du gène: RBP1
• Identification du gène (NCBI): 5947
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IHC	Metabolism; Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.; Authors - Shyi-Jang Shin
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu
rat	IHC	Brain Sci; Intraoperative Hypothermia Induces Vascular Dysfunction in the CA1 Region of Rat Hippocampus.; Authors - Tianjia Li
mouse	WB	J Food Biochem; Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.; Authors - Wei Li
human	WB,IHC,IF	Cell Death Dis; The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.; Authors - Ling Gao ; KD Validated
human	WB	FASEB J; The downregulation of HSP90-controlled CRALBP expression is associated with age-related vision attenuation; Authors - Dan-Dan Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melissa (Verified Customer) (06-13-2022)

We have used the antibody and it worked out

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1
• Cell Tissue Type: Endothelial cells

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