Anticorps Polyclonal de lapin anti-RBP2

• Phare
• Validé par KD/KO

RBP2 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18825-1-PBS

Synonymes

KDM5A, [histone H3]-trimethyl-L-lysine(4) demethylase 5A, EC:1.14.11.67, JARID1A, Jumonji/ARID domain-containing protein 1A

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Galerie de données de validation

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  WB analysis of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18825-1-AP (RBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 18825-1-AP (same clone as 18825-1-PBS)

  WB result of KDM5A antibody (18825-1-AP; 1:600; room temperature for 1.5 hours) with wild-type and KDM5A knockout HEK-293T cells. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  IP result of anti-RBP2 (IP:18825-1-AP, 4ug; Detection:18825-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using RBP2 antibody (18825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18825-1-AP (same clone as 18825-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18825-1-AP (RBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18825-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18825-1-PBS cible RBP2 dans les applications de WB, IF/ICC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lysine (K)-specific demethylase 5A
• Masse moléculaire calculée: 192 kDa
• Poids moléculaire observé: 192 kDa
• Numéro d’acquisition GenBank: NM_005056
• Symbole du gène: KDM5A
• Identification du gène (NCBI): 5927
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

KDM5A, also named as JARID1A, RBBP2 and RBP2, belongs to the JARID1 histone demethylase family. KDM5A is a Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. RBP2 may stimulate transcription mediated by nuclear receptors. It may be involved in transcriptional regulation of Hox proteins during cell differentiation. It may participate in transcriptional repression of cytokines such as CXCL12.

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