Anticorps Recombinant de lapin anti-RBP4

• Phare
• Validé par KD/KO

RBP4 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

6O13

N° de cat : 82934-1-RR

Synonymes

6O13, Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179)

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Galerie de données de validation

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  WB analysis using 82934-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82934-1-RR (RBP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 82934-1-RR

  WB result of RBP4 antibody (82934-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBP4 transfected HepG2 cells.

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  FC experiment of HepG2 using 82934-1-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (82934-1-RR, Clone:6O13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82934-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82934-1-RR against Human RBP4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, plasma humain
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82934-1-RR cible RBP4 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBP4 Protéine recombinante Ag2448
• Nom complet: retinol binding protein 4, plasma
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC020633
• Symbole du gène: RBP4
• Identification du gène (NCBI): 5950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

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