Anticorps Recombinant de lapin anti-RBP4

RBP4 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241944B3

N° de cat : 84496-1-PBS

Synonymes

241944B3, Plasma retinol binding protein, Plasma retinol-binding protein, Plasma retinol-binding protein(1-176), Plasma retinol-binding protein(1-179)

---

Galerie de données de validation

• 

  WB analysis of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 84496-1-RR (RBP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01338-1

  Sandwich ELISA standard curve of MP01338-1, Human RBP4 Recombinant Matched Antibody Pair - PBS only. 84496-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31642. 84496-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

• 

  Sample test of MP01338-1

  Serum of twelve individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 87,804.85 ng/mL with a range of 37,160.99 - 156,178.64 ng/mL.

• 

  Sample test of MP01338-1

  Urine of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 90.68 ng/mL with a range of 29.96 - 290.83 ng/mL.

• 

  Sample test of MP01338-1

  Saliva of six individual healthy human donors was measured. The RBP4 concentration of detected samples was determined to be 12.14 ng/mL with a range of 8.34-17.17 ng/mL.

• 

  Sample test of MP01338-1

  HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human RBP4, and measured 707.54 ng/mL.

• 

  FC experiment of HepG2 using 84496-1-RR (same clone as 84496-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RBP4 Recombinant antibody (84496-1-RR, Clone:241944B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84496-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01338-1

  Cytometric bead array standard curve of MP01338-1, RBP4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84496-1-PBS. Detection antibody: 84496-2-PBS. Standard: Eg31642. Range: 0.156-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 84496-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 84496-1-PBS against Human RBP4 were performed. The affinity constant is 0.504 nM.

• 

  Affinity and Kinetic Characterization of 84496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84496-1-RR against Human RBP4 were performed. The affinity constant is 0.504 nM.

---

Informations sur le produit

84496-1-PBS cible RBP4 dans les applications de WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RBP4 Protéine recombinante Eg31642
• Nom complet: retinol binding protein 4, plasma
• Masse moléculaire calculée: 201 aa, 23 kDa
• Poids moléculaire observé: 20-21 kDa
• Numéro d’acquisition GenBank: BC020633
• Symbole du gène: RBP4
• Identification du gène (NCBI): 5950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some INS-resistant states. Measurement of serum RBP4 could be used to assess the risk of INS resistance, type 2 diabetes, obesity, and cardiovascular disease. (18752671, 16034410)

{{ptg:RelatedPrimaryAntibodies}}