Anticorps Polyclonal de lapin anti-RBPJ

RBPJ Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30044-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of MCF-7 using 30044-1-AP (same clone as 30044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 30044-1-AP (same clone as 30044-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 30044-1-AP (same clone as 30044-1-PBS)

  IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug. This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 30044-1-AP (same clone as 30044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30044-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30044-1-PBS cible RBPJ dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBPJ Protéine recombinante Ag32552
• Nom complet: recombination signal binding protein for immunoglobulin kappa J region
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: ~60 kDa
• Numéro d’acquisition GenBank: BC064976
• Symbole du gène: RBPJ
• Identification du gène (NCBI): 3516
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

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