Anticorps Polyclonal de lapin anti-RC3H1

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31234-1-PBS

Synonymes

KIAA2025, RING finger protein 198, RNF198, ROQUIN

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Galerie de données de validation

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  WB analysis using 31234-1-AP (same clone as 31234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31234-1-AP (same clone as 31234-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31234-1-PBS cible RC3H1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RC3H1 Protéine recombinante Ag35089
• Nom complet: ring finger and CCCH-type zinc finger domains 1
• Masse moléculaire calculée: 126 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: NM_001300850
• Symbole du gène: RC3H1
• Identification du gène (NCBI): 149041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)

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