Anticorps Polyclonal de lapin anti-RCAN2

• Phare
• Validé par KD/KO

RCAN2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12900-1-AP

Synonymes

Calcipressin 2, CSP2, DSCR1L1, hRCN2, MCIP2, RCAN2, regulator of calcineurin 2, ZAKI 4, ZAKI4

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Galerie de données de validation

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  WB analysis using 12900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12900-1-AP

  IP result of anti-RCAN2 (IP:12900-1-AP, 4ug; Detection:12900-1-AP 1:300) with mouse brain tissue lysate 4400 ug.

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  IHC staining of human gliomas using 12900-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12900-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12900-1-AP (RCAN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 12900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 12900-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu cutané humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

12900-1-AP cible RCAN2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCAN2 Protéine recombinante Ag3597
• Nom complet: regulator of calcineurin 2
• Masse moléculaire calculée: 225 aa, 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC038509
• Symbole du gène: RCAN2
• Identification du gène (NCBI): 10231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RCAN2 is a member of the regulator of calcineurin (RCAN) protein family. These proteins play a role in many physiological processes by binding to the catalytic domain of calcineurin A, inhibiting calcineurin-mediated nuclear translocation of the transcription factor NFATC1. Expression of this gene in skin fibroblasts is upregulated by thyroid hormone, and the encoded protein may also play a role in endothelial cell function and angiogenesis.

Publications

Species	Application	Title
human	WB,IHC	Cell Biol Toxicol; USP7-mediated JUND suppresses RCAN2 transcription and elevates NFATC1 to enhance stem cell property in colorectal cancer; Authors - Yunli Chang
human	IHC,WB	Cancer Cell Int; Construction of a single nucleotide variant score-related gene-based prognostic model in hepatocellular carcinoma: analysis of multi-independent databases and validation in vitro.; Authors - Yu-Jie Xu ; KD Validated
human	WB	Int J Biol Sci; Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.; Authors - Wenbo Liu
zebrafish		PLoS Biol; The scale of zebrafish pectoral fin buds is determined by intercellular K+ levels and consequent Ca2+-mediated signaling via retinoic acid regulation of Rcan2 and Kcnk5b; Authors - Xiaowen Jiang
human	WB,IHC	Front Biosci (Landmark Ed); TMB Signature-Related RCAN2 Promotes Apoptosis by Upregulating EHF/DR5 Pathway in Hepatocellular Carcinoma; Authors - Yu-Jie Xu ; KO Validated
human,mouse	WB	Toxicol Appl Pharmacol; Knockdown of regulator of Calcineurin 2 promotes transcription factor EB-mediated lipophagy to prevent non-alcoholic fatty liver disease; Authors - Lei Shan

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