Anticorps Monoclonal anti-RCC1

RCC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C2F5

N° de cat : 67335-1-PBS

Synonymes

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

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Galerie de données de validation

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  WB analysis using 67335-1-Ig (same clone as 67335-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67335-1-Ig (same clone as 67335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67335-1-Ig (same clone as 67335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67335-1-Ig (same clone as 67335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67335-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67335-1-PBS cible RCC1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RCC1 Protéine recombinante Ag17809
• Nom complet: regulator of chromosome condensation 1
• Masse moléculaire calculée: 421 aa, 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC010067
• Symbole du gène: RCC1
• Identification du gène (NCBI): 1104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

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