Anticorps Monoclonal anti-RCHY1

• Phare
• Validé par KD/KO

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C11C11

N° de cat : 67856-1-PBS

Synonymes

PIRH2, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1

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Galerie de données de validation

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  WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

  WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67856-1-PBS cible RCHY1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RCHY1 Protéine recombinante Ag4942
• Nom complet: ring finger and CHY zinc finger domain containing 1
• Masse moléculaire calculée: 261 aa, 30 kDa
• Poids moléculaire observé: 45 KDa, 30 kDa
• Numéro d’acquisition GenBank: BC047393
• Symbole du gène: RCHY1
• Identification du gène (NCBI): 25898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

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