Anticorps Recombinant de lapin anti-RCN2

RCN2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242612A4

N° de cat : 84956-4-PBS

Synonymes

242612A4, Calcium binding protein ERC 55, Calcium-binding protein ERC-55, E6 binding protein, E6-binding protein

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Galerie de données de validation

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  WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

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  WB analysis using 84956-4-RR (same clone as 84956-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84956-4-RR (RCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84956-4-RR (same clone as 84956-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84956-4-RR (RCN2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84956-4-RR (same clone as 84956-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84956-4-RR (same clone as 84956-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RCN2 antibody (84956-4-RR, Clone: 242612A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84956-4-PBS in a different storage buffer formulation.

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Informations sur le produit

84956-4-PBS cible RCN2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RCN2 Protéine recombinante Ag0247
• Nom complet: reticulocalbin 2, EF-hand calcium binding domain
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC004892
• Symbole du gène: RCN2
• Identification du gène (NCBI): 5955
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Reticulocalbin-2 (RCN2) is also named as endoplasmic reticulum Ca2+-binding protein of 55 kDa (ERC55) and E6-binding protein (E6BP). It is strictly located in endoplasmic reticulum (PMID: 34094681). RCN2 is a low-affinity Ca2+ binding protein localized to the secretory pathway of mammalian cells, belongs to a member of the EF-hand Ca2+ binding protein family, which participate in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes (PMID: 19260022). As for cancer research, several studies showed that RCN2 was related to tumor progression, such as mammary cancer, cervical cancer, neck squamous cell carcinoma and colorectal cancer (PMID: 16351756, PMID: 32422974). RCN2 played a pivotal role in HCC pathogenesis through regulating activation of the epidermal growth factor receptor (EGFR) pathway and modulating epidermal growth factor receptor EGFR dimerization and internalization (PMID: 28745317).

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