Anticorps Polyclonal de lapin anti-RCOR2

RCOR2 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 23969-1-AP

Synonymes

CoREST2, RCOR2, REST corepressor 2

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Galerie de données de validation

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  WB analysis of Jurkat using 23969-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23969-1-AP (RCOR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23969-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23969-1-AP (RCOR2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 23969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 23969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 23969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 23969-1-AP (RCOR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293
• Résultats positifs en IHC: tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

23969-1-AP cible RCOR2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RCOR2 Protéine recombinante Ag21112
• Nom complet: REST corepressor 2
• Masse moléculaire calculée: 523 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC023587
• Symbole du gène: RCOR2
• Identification du gène (NCBI): 283248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In mammals, the CoREST (corepressor for element-1-silencing transcription factor) complex is a chromatin-modifying structure that, through interactions with NRSF (neuron restrictive silencer factor), regulates neuronal gene expression and neuronal cell fate. RCOR2 (REST corepressor 2) and RCOR3 (REST corepressor 3) are nuclear proteins that each contain one ELM2 domain and two SANT domains. RCOR2 and RCOR3, both members of the CoREST family, are thought to function as components of the CoREST complex, possibly playing a role in the transcriptional repression of neuronal genes. Additionally, RCOR2 and RCOR3, in conjunction with CoREST, can form immunocomplexes with a variety of histone-modifying genes, including G9a and HDAC1. Via these protein complexes, RCOR2 and RCOR3 can further regulate transcription by controlling the methylation and demethylation of target genes during early development.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
human	WB	Nat Commun; Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition; Authors - Cheng Zeng ; KO Validated