Anticorps Polyclonal de lapin anti-RD3

RD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14855-1-PBS

Synonymes

C1orf36, LCA12, Protein RD3, retinal degeneration 3, Retinal degeneration protein 3

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Galerie de données de validation

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  WB analysis using 14855-1-AP (same clone as 14855-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14855-1-AP (RD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IHC staining of rat eye using 14855-1-AP (same clone as 14855-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 14855-1-AP (same clone as 14855-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using RD3 antibody (14855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14855-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14855-1-PBS cible RD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RD3 Protéine recombinante Ag6641
• Nom complet: retinal degeneration 3
• Masse moléculaire calculée: 22.7 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC065541
• Symbole du gène: RD3
• Identification du gène (NCBI): 343035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RD3, or Retinal Degeneration 3, plays a critical role in regulating guanylate cyclase (GC) signaling and photoreceptor cell survival (PMID: 30559291). RD3 is highly conserved across vertebrates, with the human protein sharing high sequence identity with other primates and varying degrees of identity with other species (PMID: 29030614). The main functions of RD3 include inhibiting photoreceptor-specific guanylate cyclase activity and promoting the accumulation of retinal membrane guanylyl cyclase (RetGC) in the photoreceptor outer segment (PMID: 30559291). RD3 is essential for the normal expression of RetGC in photoreceptor cells and blocks RetGC catalytic activity. Mutations in the RD3 gene can lead to Leber congenital amaurosis type 12, which results in retinal degeneration. RD3 is also involved in the trafficking of RetGC from the endoplasmic reticulum to the photoreceptor outer segments, which is crucial for maintaining the normal function and survival of photoreceptors (PMID: 34537244).

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