Anticorps Recombinant de lapin anti-RDBP

RDBP Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250420G2

N° de cat : 85945-1-PBS

Synonymes

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

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Galerie de données de validation

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  WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

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  WB analysis using 85945-1-RR (same clone as 85945-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85945-1-RR (same clone as 85945-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85945-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85945-1-PBS cible RDBP dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RDBP Protéine recombinante Ag1186
• Nom complet: RD RNA binding protein
• Masse moléculaire calculée: 37 kDa, 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC011600
• Symbole du gène: NELFE
• Identification du gène (NCBI): 7936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

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