Anticorps Recombinant de lapin anti-RDBP

RDBP Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250420G2

N° de cat : 85945-1-RR

Synonymes

NELFE, NELF E, NELF-E, RD, RNA-binding protein RD

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Galerie de données de validation

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  WB analysis using 85945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85945-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85945-1-RR (RDBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85945-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RDBP antibody (85945-1-RR, Clone: 250420G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2500-1:7500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85945-1-RR cible RDBP dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RDBP Protéine recombinante Ag1186
• Nom complet: RD RNA binding protein
• Masse moléculaire calculée: 37 kDa, 43 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC011600
• Symbole du gène: NELFE
• Identification du gène (NCBI): 7936
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

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