Anticorps Polyclonal de lapin anti-RDM1

• Phare
• Validé par KD/KO

RDM1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20156-1-AP

Synonymes

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

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Galerie de données de validation

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  WB analysis of PC-3 using 20156-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20156-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20156-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20156-1-AP (RDM1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 20156-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20156-1-AP (RDM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of PC-3 using 20156-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20156-1-AP (RDM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7, tissu placentaire humain
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20156-1-AP cible RDM1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RDM1 Protéine recombinante Ag14077
• Nom complet: RAD52 motif 1
• Masse moléculaire calculée: 284 aa, 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC038301
• Symbole du gène: RDM1
• Identification du gène (NCBI): 201299
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

Publications

Species	Application	Title
human	WB,IHC,IF	Mol Oncol; Loss of RDM1 enhances hepatocellular carcinoma progression via p53 and Ras/Raf/ERK pathways.; Authors - Shi-Lu Chen ; KD Validated
human	WB	J Cell Mol Med; RDM1 promotes critical processes in breast cancer tumorigenesis.; Authors - Yajun Chen ; KD Validated
human	WB	Front Cell Dev Biol; A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.; Authors - Zewei Tu
human	WB,IHC	Artif Cells Nanomed Biotechnol; RDM1 plays an oncogenic role in human ovarian carcinoma cells.; Authors - Lu Tong ; KD Validated
human	WB,IHC	Sci Rep; RDM1 plays an oncogenic role in human lung adenocarcinoma cells.; Authors - Lu Tong ; KD Validated
human	WB,IHC	FEBS Open Bio; RDM1 promotes neuroblastoma growth through the RAS-Raf-MEK-ERK pathway.; Authors - Guojin Xie ; KD Validated