Anticorps Monoclonal anti-SLC1A5/ASCT2

SLC1A5/ASCT2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1C4E1

N° de cat : 68540-1-Ig

Synonymes

SLC1A5, 1C4E1, AAAT, ASCT2, ATB(0)

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Galerie de données de validation

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  WB analysis using 68540-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68540-1-Ig (SLC1A5/ASCT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 68540-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 68540-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68540-1-Ig (RDRC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 68540-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of HEK-293 using 68540-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (68540-1-Ig, Clone: 1C4E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 68540-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (68540-1-Ig, Clone:1C4E1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HT-29
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-Fro: tissu d'intestin grêle de souris,
• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68540-1-Ig cible SLC1A5/ASCT2 dans les applications de WB, IHC, IF/ICC, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC1A5/ASCT2 Protéine recombinante Ag16837
• Nom complet: solute carrier family 1 (neutral amino acid transporter), member 5
• Masse moléculaire calculée: 541 aa, 57 kDa
• Poids moléculaire observé: 55-70 kDa
• Numéro d’acquisition GenBank: BC000062
• Symbole du gène: SLC1A5/ASCT2
• Identification du gène (NCBI): 6510
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).

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