Anticorps Monoclonal anti-REDD1

• Phare
• Validé par KD/KO

REDD1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A2C10

N° de cat : 67059-1-PBS

Synonymes

DDIT4, 3A2C10, DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, REDD-1

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Galerie de données de validation

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  WB analysis using 67059-1-Ig (same clone as 67059-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67059-1-Ig (same clone as 67059-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67059-1-Ig (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67059-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67059-1-PBS cible REDD1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: REDD1 Protéine recombinante Ag0965
• Nom complet: DNA-damage-inducible transcript 4
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC007714
• Symbole du gène: REDD1/DDIT4
• Identification du gène (NCBI): 54541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].

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