Anticorps Recombinant de lapin anti-REDD1

REDD1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1L2

N° de cat : 82650-1-RR

Synonymes

DDIT4, 1L2, RTP801, REDD-1, HIF-1 responsive protein RTP801

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Galerie de données de validation

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  WB analysis using 82650-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 82650-1-RR

  Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 82650-1-RR (REDD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82650-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82650-1-RR against Human REDD1 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa traitées au chlorure de cobalt, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82650-1-RR cible REDD1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: REDD1 Protéine recombinante Ag0965
• Nom complet: DNA-damage-inducible transcript 4
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC007714
• Symbole du gène: REDD1/DDIT4
• Identification du gène (NCBI): 54541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].

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