Anticorps Polyclonal de lapin anti-REEP2

• Phare
• Validé par KD/KO

REEP2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15684-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15684-1-AP (same clone as 15684-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 15684-1-AP (same clone as 15684-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 15684-1-AP (same clone as 15684-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 15684-1-AP (same clone as 15684-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 15684-1-AP (same clone as 15684-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15684-1-AP (REEP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 15684-1-AP (same clone as 15684-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug REEP2 Polyclonal antibody (15684-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15684-1-PBS cible REEP2 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: REEP2 Protéine recombinante Ag8186
• Nom complet: receptor accessory protein 2
• Masse moléculaire calculée: 252 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC006218
• Symbole du gène: REEP2
• Identification du gène (NCBI): 51308
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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