Anticorps Polyclonal de lapin anti-RENALASE

• Phare
• Validé par KD/KO

RENALASE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15003-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15003-1-AP (same clone as 15003-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15003-1-AP (RENALASE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15003-1-AP (same clone as 15003-1-PBS)

  IP result of anti-RENALASE (IP:15003-1-AP, 4ug; Detection:15003-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15003-1-AP (same clone as 15003-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 15003-1-AP (same clone as 15003-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15003-1-AP (RENALASE antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 15003-1-AP (same clone as 15003-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using RENALASE antibody (15003-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 15003-1-AP (same clone as 15003-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 15003-1-AP (RENALASE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15003-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15003-1-PBS cible RENALASE dans les applications de WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RENALASE Protéine recombinante Ag13061
• Nom complet: chromosome 10 open reading frame 59
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC005364
• Symbole du gène: RENALASE
• Identification du gène (NCBI): 55328
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.

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