Anticorps Polyclonal de lapin anti-RERG

• Phare
• Validé par KD/KO

RERG Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10687-1-AP

Synonymes

RERC, RERG

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Galerie de données de validation

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  WB analysis of MCF-7 using 10687-1-AP

  WB result of RERG antibody (10687-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RERG transfected MCF-7 cells.

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  WB analysis using 10687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10687-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10687-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10687-1-AP cible RERG dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RERG Protéine recombinante Ag1041
• Nom complet: RAS-like, estrogen-regulated, growth inhibitor
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23-26 kDa
• Numéro d’acquisition GenBank: BC007997
• Symbole du gène: RERG
• Identification du gène (NCBI): 85004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RERG (Ras-related and estrogen-regulated growth inhibitor), a gene that encodes a small GTP binding and hydrolyzing protein (GTPase) of the Ras superfamily, is a novel gene that was first reported in breast cancer. RERG gene expression is stimulated by the estrogen receptor alpha (ERalpha), and RERG gene expression is absent in ER-negative breast cancer. RERG can bind and hydrolyze GTP as a GTP/GDP molecular switch, and RERG has been shown to possess growth inhibitory activity in breast cancer cells.

Publications

Species	Application	Title
human	WB,IF	Front Oncol; The NFAT3/RERG Complex in Luminal Breast Cancers Is Required to Inhibit Cell Invasion and May Be Correlated With an Absence of Axillary Lymph Nodes Colonization.; Authors - Lucie Coillard ; KD Validated
human	IHC	Breast Cancer Res Treat; RERG (Ras-like, oestrogen-regulated, growth-inhibitor) expression in breast cancer: a marker of ER-positive luminal-like subtype.; Authors - Habashy Hany Onsy HO
		Oncol Rep; ERK5‑regulated RERG expression promotes cancer progression in prostatic carcinoma.; Authors - Ying Xiong
human	WB,IHC	Oncotarget; MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis.; Authors - Jar-Yi Ho
human	IHC	Front Med (Lausanne); Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL; Authors - Xujuan Zhang