Anticorps Monoclonal anti-RFC4

RFC4 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A6A11

N° de cat : 68390-1-PBS

Synonymes

RFC37, RF-C 37 kDa subunit, A1, 1A6A11

---

Galerie de données de validation

• 

  WB analysis using 68390-1-Ig (same clone as 68390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68390-1-Ig (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68390-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 68390-1-Ig (same clone as 68390-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug RFC4 Monoclonal antibody (68390-1-Ig, Clone:1A6A11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68390-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68390-1-PBS cible RFC4 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RFC4 Protéine recombinante Ag33295
• Nom complet: replication factor C (activator 1) 4, 37kDa
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC017452
• Symbole du gène: RFC4
• Identification du gène (NCBI): 5984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}