Anticorps Polyclonal de lapin anti-RFC5

RFC5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28671-1-PBS

Synonymes

A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit

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Galerie de données de validation

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  WB analysis using 28671-1-AP (same clone as 28671-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28671-1-AP (RFC5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28671-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28671-1-AP (same clone as 28671-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28671-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28671-1-AP (same clone as 28671-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28671-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28671-1-PBS cible RFC5 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFC5 Protéine recombinante Ag29236
• Nom complet: replication factor C (activator 1) 5, 36.5kDa
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC001866
• Symbole du gène: RFC5
• Identification du gène (NCBI): 5985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Replication factor C subunit 5 (RFC5), also known as activator 1, can recruit proliferating cell nuclear antigen(PCNA) onto the DNA polymerase S-phase checkpoint complex. In eukaryotes, RFC5 is involved in repairing mismatches, DNA double helix damage, nucleotide excision, and regulating the cell cycle(PMID: 30210909). RFC5 is significantly upregulated in cancer tissues or cells, and its expression is elevated with the cancer progression(PMID: 30214556).

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