Anticorps Polyclonal de lapin anti-RFTN1

• Phare
• Validé par KD/KO

RFTN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24289-1-PBS

Synonymes

Raftlin, PIG9, PIB10, MIG2, KIAA0084

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Galerie de données de validation

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  WB analysis of HeLa using 24289-1-AP (same clone as 24289-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 24289-1-AP (same clone as 24289-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24289-1-PBS cible RFTN1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFTN1 Protéine recombinante Ag17264
• Nom complet: raftlin, lipid raft linker 1
• Masse moléculaire calculée: 578 aa, 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC100667
• Symbole du gène: RFTN1
• Identification du gène (NCBI): 23180
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).

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