Anticorps Polyclonal de lapin anti-RFWD3

• Phare
• Validé par KD/KO

RFWD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19893-1-AP

Synonymes

RFWD3, RING finger protein 201, RNF201

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19893-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human testis using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19893-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HEK-293 using 19893-1-AP

  Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 19893-1-AP

  Immunofluorescent analysis of Hela cells, using RFWD3 antibody 19893-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:200
• Immunofluorescence (IF): IF : 1:20-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

19893-1-AP cible RFWD3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFWD3 Protéine recombinante Ag13728
• Nom complet: ring finger and WD repeat domain 3
• Masse moléculaire calculée: 774 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC002574
• Symbole du gène: RFWD3
• Identification du gène (NCBI): 55159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DOCK7 protects against replication stress by promoting RPA stability on chromatin.; Authors - Ming Gao
human	WB,IF	Front Oncol; The Valproate Mediates Radio-Bidirectional Regulation Through RFWD3-Dependent Ubiquitination on Rad51.; Authors - Guochao Liu ; KD Validated
human	IHC	Front Med; Identification of an E3 ligase-encoding gene RFWD3 in non-small cell lung cancer.; Authors - Yanfei Zhang
human	IHC	Histol Histopathol; RFWD3 acts as a tumor promotor in the development and progression of bladder cancer; Authors - Peng Jiang