Anticorps Polyclonal de lapin anti-RFWD3

• Phare
• Validé par KD/KO

RFWD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19893-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse lung using 19893-1-AP (same clone as 19893-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 19893-1-AP (same clone as 19893-1-PBS)

  Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19893-1-PBS cible RFWD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFWD3 Protéine recombinante Ag13728
• Nom complet: ring finger and WD repeat domain 3
• Masse moléculaire calculée: 774 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC002574
• Symbole du gène: RFWD3
• Identification du gène (NCBI): 55159
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.

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