Anticorps Monoclonal anti-RFX1

RFX1 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G10H6

N° de cat : 68057-1-PBS

Synonymes

RFX, EF-C, EF C, 1G10H6

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Galerie de données de validation

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  WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 68057-1-Ig (same clone as 68057-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68057-1-Ig (same clone as 68057-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 68057-1-Ig (same clone as 68057-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68057-1-PBS cible RFX1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RFX1 Protéine recombinante Ag25442
• Nom complet: regulatory factor X, 1 (influences HLA class II expression)
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC049826
• Symbole du gène: RFX1
• Identification du gène (NCBI): 5989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

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