Anticorps Polyclonal de lapin anti-RFX2

RFX2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12622-1-PBS

Synonymes

DNA binding protein RFX2, DNA-binding protein RFX2, Regulatory factor X 2

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Galerie de données de validation

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  WB analysis using 12622-1-AP (same clone as 12622-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12622-1-AP (RFX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 12622-1-AP (same clone as 12622-1-PBS)

  IP result of anti-RFX2 (IP:12622-1-AP, 4ug; Detection:12622-1-AP 1:1000) with THP-1 cells lysate 1920 ug. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12622-1-PBS cible RFX2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RFX2 Protéine recombinante Ag3310
• Nom complet: regulatory factor X, 2 (influences HLA class II expression)
• Masse moléculaire calculée: 723 aa, 80 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC028579
• Symbole du gène: RFX2
• Identification du gène (NCBI): 5990
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RFX2 is a key regulator of ciliogenesis in vertebrates, and knockdown of Rfx2 causes defects in neural tube closure and in left-right axis patterning (PMID: 26248850). RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids(PMID: 22227339). RFX2 is also required for the development of left-right asymmetry, and motile cilia on RFX2-expressing cells are sufficient to establish adequate fluid flow across the COA to direct normal LR development in mouse (PMID: 22233545).

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