Anticorps Polyclonal de lapin anti-RGC32

• Phare
• Validé par KD/KO

RGC32 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19556-1-PBS

Synonymes

C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32

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Galerie de données de validation

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  WB analysis using 19556-1-AP (same clone as 19556-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19556-1-AP (RGC32 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19556-1-AP (same clone as 19556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19556-1-AP (same clone as 19556-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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  IF Staining of Caco-2 using 19556-1-AP (same clone as 19556-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using RGC32 antibody (19556-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19556-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19556-1-PBS cible RGC32 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: chromosome 13 open reading frame 15
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: NM_014059
• Symbole du gène: RGC32
• Identification du gène (NCBI): 28984
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.

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