Anticorps Polyclonal de lapin anti-RGR

RGR Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11904-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human heart using 11904-1-AP (same clone as 11904-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11904-1-AP (RGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 11904-1-AP (same clone as 11904-1-PBS)

  IP result of anti-RGR (IP:11904-1-AP, 4ug; Detection:11904-1-AP 1:300) with PC-3 cells lysate 4000ug. This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse retina using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse retina using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11904-1-AP (RGR antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11904-1-PBS cible RGR dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGR Protéine recombinante Ag2552
• Nom complet: retinal G protein coupled receptor
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa, 26 kDa
• Numéro d’acquisition GenBank: BC008094
• Symbole du gène: RGR
• Identification du gène (NCBI): 5995
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RGR is a G protein-coupled receptor preferentially expressed at high levels in the retinal pigment epithelium (RPE) and Mueller cells of the neural retina. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. RGR acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The gene of PGR may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.

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