Anticorps Polyclonal de lapin anti-RGS1

• Phare
• Validé par KD/KO

RGS1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12547-1-PBS

Synonymes

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

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Galerie de données de validation

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  WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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  WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 12547-1-AP (same clone as 12547-1-PBS)

  WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12547-1-AP (same clone as 12547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12547-1-PBS cible RGS1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS1 Protéine recombinante Ag3315
• Nom complet: regulator of G-protein signaling 1
• Masse moléculaire calculée: 196 aa, 22 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC015510
• Symbole du gène: RGS1
• Identification du gène (NCBI): 5996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).

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