Anticorps Polyclonal de lapin anti-RGS12

RGS12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29803-1-PBS

Synonymes

Regulator of G-protein signaling 12

---

Galerie de données de validation

• 

  WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat cerebellum using 29803-1-AP (same clone as 29803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

• 

  IF Staining of PC-3 using 29803-1-AP (same clone as 29803-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

29803-1-PBS cible RGS12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS12 Protéine recombinante Ag30731
• Nom complet: regulator of G-protein signaling 12
• Masse moléculaire calculée: 156 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_198229
• Symbole du gène: RGS12
• Identification du gène (NCBI): 6002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.

{{ptg:RelatedPrimaryAntibodies}}