Anticorps Recombinant de lapin anti-RGS12

RGS12 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242530G11

N° de cat : 85057-2-PBS

Synonymes

242530G11

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Galerie de données de validation

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  WB analysis using 85057-2-RR (same clone as 85057-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85057-2-RR (RGS12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01790-2

  Sandwich ELISA standard curve of MP01790-2, Human RGS12 Recombinant Matched Antibody Pair - PBS only. 85057-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30731. 85057-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01790-2

  The mean RGS12 concentration was determined to be 18,450.2 pg/mL in A431 cell extract based on a 1.3 mg/mL extract load, 6,327.2 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 42,390.5 pg/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

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  IF Staining of PC-3 using 85057-2-RR (same clone as 85057-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using RGS12 antibody (85057-2-RR, Clone: 242530G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01790-2

  Cytometric bead array standard curve of MP01790-2, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-3-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 85057-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85057-2-RR against Human RGS12 were performed. The affinity constant is 0.611 nM.

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Informations sur le produit

85057-2-PBS cible RGS12 dans les applications de WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RGS12 Protéine recombinante Ag30731
• Nom complet: regulator of G-protein signaling 12
• Masse moléculaire calculée: 156 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_198229
• Symbole du gène: RGS12
• Identification du gène (NCBI): 6002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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