Anticorps Monoclonal anti-RGS3

RGS3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1D6A3

N° de cat : 66790-1-Ig

Synonymes

C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ RGS3, RGP3, RGS3

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Galerie de données de validation

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  WB analysis of Jurkat using 66790-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66790-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 66790-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RGS3 antibody (66790-1-Ig, Clone: 1D6A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66790-1-Ig cible RGS3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RGS3 Protéine recombinante Ag3177
• Nom complet: regulator of G-protein signaling 3
• Masse moléculaire calculée: 1198 aa, 132 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC018072
• Symbole du gène: RGS3
• Identification du gène (NCBI): 5998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RGS3 belongs to a family of proteins which share the core regulator of G-protein signalling (RGS) domain and function as GTPase-activating proteins (GAPs) for the α subunit of heterotrimeric G-proteins. RGS3 has been reported to inhibit the Gai-and Gaq-mediated signaling pathways in the cardiovascular system, including those acting via the cardiovascular signaling molecules angiotensin II (Ang II), sphingosine 1-phosphate and endothelin-1. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear.

Publications

Species	Application	Title
mouse	WB	Transl Cancer Res; Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1; Authors - Xiao Hu
human	WB	Stem Cell Res Ther; Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy; Authors - Xuehui Fan

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