Anticorps Polyclonal de lapin anti-RGS5

• Phare
• Validé par KD/KO

RGS5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11590-1-PBS

Synonymes

Regulator of G-protein signaling 5

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Galerie de données de validation

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  WB analysis using 11590-1-AP (same clone as 11590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 11590-1-AP (same clone as 11590-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 11590-1-AP (same clone as 11590-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 11590-1-AP (same clone as 11590-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11590-1-AP (same clone as 11590-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 11590-1-AP (same clone as 11590-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11590-1-AP (same clone as 11590-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 11590-1-AP (same clone as 11590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 11590-1-AP (same clone as 11590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11590-1-AP (same clone as 11590-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RGS5 antibody (11590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11590-1-AP (same clone as 11590-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RGS5 (11590-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11590-1-PBS cible RGS5 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RGS5 Protéine recombinante Ag2196
• Nom complet: regulator of G-protein signaling 5
• Masse moléculaire calculée: 181 aa, 21 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC030059
• Symbole du gène: RGS5
• Identification du gène (NCBI): 8490
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)

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