Anticorps Polyclonal de lapin anti-RHBDD1

• Phare
• Validé par KD/KO

RHBDD1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 20869-1-AP

Synonymes

HSD 50, RHBDD1, RHBDL4, rhomboid domain containing 1

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Galerie de données de validation

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  WB analysis of mouse kidney using 20869-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20869-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20869-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20869-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 20869-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 20869-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 20869-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules PC-3, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below

Informations sur le produit

20869-1-AP cible RHBDD1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHBDD1 Protéine recombinante Ag14932
• Nom complet: rhomboid domain containing 1
• Masse moléculaire calculée: 315 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC027900
• Symbole du gène: RHBDD1
• Identification du gène (NCBI): 84236
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4.; Authors - Koji Shibuya
human	WB	Environ Toxicol; Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway.; Authors - Jiong Jiang ; KD Validated
human	WB	Cell Death Dis; Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer.; Authors - Xiaohui Zhang
human	WB	Genes Cells; Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation; Authors - Shinichiro Yamamoto ; KD Validated

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