Anticorps Monoclonal anti-RHOA

• Phare
• Validé par KD/KO

RHOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3D7

N° de cat : 66733-1-PBS

Synonymes

ARH12, ARHA, RHO12, 1B3D7, EC:3.6.5.2

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Galerie de données de validation

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  WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66733-1-PBS cible RHOA dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RHOA Protéine recombinante Ag1141
• Nom complet: ras homolog gene family, member A
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC005976
• Symbole du gène: RHOA
• Identification du gène (NCBI): 387
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

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